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Western blot analysis of Syntaphilin in rat brain tissue lysate with Syntaphilin antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of Syntaphilin in human brain with Syntaphilin antibody at 5 μg/mL.
Immunofluorescence of Syntaphilin in Human Brain cells with Syntaphilin antibody at 20 μg/mL.
Western blot analysis of Syntaphilin in rat brain tissue lysate with Syntaphilin antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

Syntaphilin Antibody

CATALOG NUMBER: 4635

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Syntaphilin antibody was raised against a 16 amino acid synthetic peptide from near the center of human Syntaphilin.
The immunogen is located within amino acids 350 - 400 of Syntaphilin.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
Syntaphilin antibody can be used for detection of Syntaphilin by Western blot at 1 - 2 μg/mL. Despite its predicted molecular weight, Syntaphilin usually migrates at higher molecular weight in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1463 - Rat Brain Tissue Lysate
Positive Control 2:
Cat. No. 10-301 - Human Brain Tissue Slide
Purification:
Syntaphilin Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Syntaphilin Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
Syntaphilin antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SNPH
Additional Names:
Syntaphilin Antibody: bA314N13.5, KIAA0374, Syntaphilin
Protein Accession Number:
O15079
Protein Gi Number:
21362912
Ncbi Gene Id Number:
9751
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Syntaphilin Antibody: Syntaphilin was initially identified in a yeast two-hybrid screen with the carboxy terminal region of Syntaxin-1 as bait. Syntaxin-1 is a key component of the synaptic vesicle docking machinery that forms the SNARE complex with synaptobrevin and SNAP-25. Syntaphilin competes with SNAP-25 for binding to syntaxin-1 and inhibits the formation of the SNARE complex, thereby potentially regulating synaptic vesicle exocytosis. Syntaphilin also binds dynamin-1 and inhibits dynamin-dependent endocytosis. Mice lacking syntaphilin show an increased level of mitochondrial motility and a reduced density of axonal mitochondria. This correlates with an enhanced short-term facilitation and significant impairments in motor ability, suggesting syntaphilin plays a major role in presynaptic function. Multiple isoforms are known to exist.
Background Reference 1:
Lao G, Scheuss V, Gerwin CM, et al. Syntaphilin: a syntaxin-1 clamp that controls SNARE assembly. Neuron2000; 25:191-201.
Background Reference 2:
Sorensen JB. SNARE complexes prepare for membrane fusion. Trends Neurosci.2005; 28:453-5.
Background Reference 3:
Das S, Gerwin C, and Sheng ZH. Syntaphilin binds to dynamin-1 and inhibits dynamin-dependent endocytosis. J. Biol. Chem.2003; 278:41221-6.
Background Reference 4:
Kang J-S, Tian J-H, Pan P-Y, et al. Docking of axonal mitochondria by syntaphilin controls their mobility and affects short-term facilitation. Cell2008; 132:137-148.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4635

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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