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46-460 (4ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
46-460 (4ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Syntenin 2 Antibody

CATALOG NUMBER: 46-460

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat
Immunogen:
The immunogen for this antibody is: C-LLHHTMDHSIPDA
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave no signal but low background in Human Brain and Liver lysates at up to 1ug/ml. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?Immunohistochemistry:In paraffin embedded Human Liver shows staining of cytoplasm in hepatocytes. Recommended concentration, 4-6ug/ml.
Specificity:
This antibody is expected to recognise both human isoforms (NP_056500; NP_536737).
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
SDCBP2
Additional Names:
SDCBP2, ST-2, SITAC18, syndecan binding protein (syntenin) 2, syntenin-2, FLJ12256, OTTHUMP00000029961, OTTHUMP00000029964, syndecan binding protein 2
Protein Accession Number:
NP_056500.3,NP_536737.3
Protein Gi Number:
38016916
Ncbi Gene Id Number:
27111
Background Reference 1:
Koroll M, Rathjen FG, Volkmer H. The neural cell recognition molecule neurofascin interacts with syntenin-1 but not with syntenin-2, both of which reveal self-associating activity. J Biol Chem. 2001 Apr 6;276(14):10646-54.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-460

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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