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46-459 (0.01µg/ml) staining of Human HEK293 cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
46-459 (0.01µg/ml) staining of Human HEK293 cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Syntenin Antibody

CATALOG NUMBER: 46-459

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: SLEDLKVDKVIQAQ-C
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 28-30kDa band observed in lysates of cell lines HEK293 and A431 (calculated MW of 32.4kDa according to NP_005616.2 and NP_001007068.1 and of 32.3kDa according to NP_001007070.1 and NP_001007071.1). Recommended concentration: 0.01-0.03µg/ml. Primary incubation was 1 hour. IHC: Paraffin embedded Human Breast. Recommended concentration: 10µg/ml.
Specificity:
This antibody is expected to recognise isoform 1 (NP_005616.2 and NP_001007068.1) and isoform 3 (NP_001007070.1and NP_001007071.1) only.
Positive Control 1:
Cat. No. 1210 - 293 Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
SDCBP
Additional Names:
SDCBP, Syntenin, syndecan binding protein (syntenin), MDA-9, ST1, SYCL, TACIP18 , melanoma differentiation associated protein-9, pro-TGF-alpha cytoplasmic domain-interacting protein 18, scaffold protein Pbp1 syntenin
Protein Accession Number:
NP_005616.2,NP_001007068.1,NP_001007069,NP_001007070.1,NP_001007071.1
Protein Gi Number:
56243522
Ncbi Gene Id Number:
6386
Background Reference 1:
Latysheva N, Muratov G, Rajesh S, Padgett M, Hotchin NA, Overduin M, Berditchevski F. Syntenin-1 is a new component of tetraspanin-enriched microdomains: mechanisms and consequences of the interaction of syntenin-1 with CD63. Mol Cell Biol. 2006 Aug 14;

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-459

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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