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46-462 (0.3ug/ml) staining of Human Duodenum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
46-462 (0.3ug/ml) staining of Human Duodenum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Syntrophin gamma 2 Antibody

CATALOG NUMBER: 46-462

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-DSQSLARKYMYSS
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 70kDa band observed in Human Duodenum and Human Ileum lysates (calculated MW of 60.2kDa according to NP_061841.1). Recommended concentration: 0.1-0.3ug/ml.An additional band of unknown identity was also consistently observed at 110kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Positive Control 1:
Cat. No. 1364 - Human Duodenum Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
SNTG2
Additional Names:
SNTG2, SYN5, G2SYN, syntrophin, gamma 2, syntrophin 5 gamma2-syntrophin, MGC133174, syntrophin 5, SYN5, gamma2-syntrophin
Protein Accession Number:
NP_061841.2
Protein Gi Number:
259155293
Ncbi Gene Id Number:
54221
Background Reference 1:
Piluso G, Mirabella M, Ricci E, Belsito A, Abbondanza C, Servidei S, Puca AA, Tonali P, Puca GA, Nigro V. Gamma1- and gamma2-syntrophins, two novel dystrophin-binding proteins localized in neuronal cells. J Biol Chem. 2000 May 26;275(21):15851-60.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-462

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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