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Western blot analysis of SYPL1 in human brain tissue lysate with SYPL1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Western blot analysis of SYPL1 in human brain tissue lysate with SYPL1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Trial Size

SYPL1 Antibody

CATALOG NUMBER: 5285

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
SYPL1 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human SYPL1.
The immunogen is located within the first 50 amino acids of SYPL1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
SYPL1 antibody can be used for detection of SYPL1 by Western blot at 1 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Specificity:
This antibody will not recognize synaptophysin of SYPL2. Two isoforms of SYPL1 are known to exist; this antibody will only recognize the longer isoform.
Positive Control 1:
Cat. No. 1303 - Human Brain Tissue Lysate
Purification:
SYPL1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SYPL1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SYPL1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SYPL1
Additional Names:
SYPL1 Antibody: SYPL, H-SP1, SYPL, Synaptophysin-like protein 1, Pantophysin
Protein Accession Number:
NP_006745
Protein Gi Number:
5803185
Ncbi Gene Id Number:
6856
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SYPL1 Antibody: The synaptophysin-like protein 1, also known as pantophysin, is a homolog of the neuroendocrine-specific protein synaptophysin, with the highest level of homology across its four transmembrane domains. Unlike synaptophysin however, SYPL1 is ubiquitously expressed and found in small cytoplasmic transport vesicles regardless of their content. SYPL1 is thought to play a multifunctional role in vesicle biogenesis and transport and is a component of adipocyte transport vesicles, and thus may be involved in adipocyte secretion. SYPL1 also interacts with vesicle-associated membrane protein 2 (VAMP-2) in adipocytes and associates with GLUT4-containing vesicles.
Background Reference 1:
Haass NK, Kartenbeck MA, and Leube RE. Pantophysin is a ubiquitously expressed synaptophysin homologue and defines constitutive transport vesicles. J. Cell Biol.1996; 134:731-46.
Background Reference 2:
Brooks CC, Scherer PE, Cleveland K, et al. Pantophysin is a phosphoprotein component of adipocyte transport vesicles and associates with GLUT4-containin vesicles. J. Biol. Chem.2000; 275:2029-36.
Background Reference 3:
Bradley RL, Cleveland KA, and Cheatham B. The adipocyte as a secretory organ: mechanism of vesicle transport and secretory pathways. Recent Prog. Horm. Res.2001; 56:329-58.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5285

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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