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Western blot analysis of SYPL2 in human spleen tissue lysate with SYPL2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of SYPL2 in human spleen tissue with SYPL2 antibody at 2.5 μg/mL.
Immunofluorescence of SYPL2 in Human Spleen cells with SYPL2 antibody at 20 μg/mL.
Western blot analysis of SYPL2 in human spleen tissue lysate with SYPL2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Trial Size

SYPL2 Antibody

CATALOG NUMBER: 5255

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Rabbit: (100%)
Immunogen:
SYPL2 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human SYPL.
The immunogen is located within the last 50 amino acids of SYPL2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
SYPL2 antibody can be used for detection of SYPL2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
At least two isoforms of SYPL2 are known to exist. SYPL2 antibody will not cross-react with SYPL1.
Positive Control 1:
Cat. No. 1306 - Human Spleen Tissue Lysate
Positive Control 2:
Cat. No. 10-901 - Human Spleen Tissue Slide
Purification:
SYPL2 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SYPL2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SYPL2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SYPL2
Additional Names:
SYPL2 Antibody: MG29, Synaptophysin-like protein 2
Protein Accession Number:
NP_001035799
Protein Gi Number:
105554421
Ncbi Gene Id Number:
284612
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SYPL2 Antibody: SYPL2, also known as Mitsugumin 29, was initially identified as a transmembrane protein from the triad junction in skeletal muscle that had significant homology with members of the synaptophysin family. SYPL2 is thought to participate in the excitation-contraction coupling process of skeletal muscle as SYPL2-null mice showed reduced muscle contractile force and altered triad junction structure and increased susceptibility to fatigue of the skeletal muscle. SYPL2 plays a critical role in muscle Ca2+ signaling by regulating the process of store-operated Ca2+ entry and interacts with ryanodine receptor (RyR), thereby influencing intracellular Ca2+ homeostasis through changes in the RyR/Ca2+ release function. Co-expression of SYPL2 and RyR in cultured cells leads to apoptotic cell death resulting from the depletion of Ca2+ from the intracellular stores.
Background Reference 1:
Takeshima H, Simuta M, Komazaki S, et al. Mitsugumin29, a novel synaptophysin family member from the triad junction in skeletal muscle. Biochem. J.1998; 331:317-22.
Background Reference 2:
Nishi M, Komazaki S, Kurebayashi Y, et al. Abnormal features in skeletal muscle from mice lacking mitsugumin29. J. Cell Biol.1999; 147:1473-80.
Background Reference 3:
Nagaraj RY, Nosek CM, Brotto MA, et al. Increased susceptibility to fatigue of slow- and fast-twitch muscles from mice lacking the MG29 gene. Physiol. Genom.2000; 4:43-9.
Background Reference 4:
Pan Z, Hirata D, Nagaraj RY, et al. Dysfunction of store-operated calcium channel in muscle cells lacking mg29. Nat. Cell Biol.2002; 4:379-83.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5255

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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