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Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
SYT1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).

SYT1 Antibody

CATALOG NUMBER: 57-214

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Chicken, Monkey, Mouse, Rat
Immunogen:
This SYT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 349-377 amino acids from the C-terminal region of human SYT1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
48 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SYT1
Additional Names:
Synaptotagmin-1, Synaptotagmin I, SytI, p65, SYT1, SVP65, SYT
Protein Accession Number:
P21579
Protein Gi Number:
135086
Ncbi Gene Id Number:
6857
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The synaptotagmins are integral membrane proteins ofsynaptic vesicles thought to serve as Ca(2+) sensors in the processof vesicular trafficking and exocytosis. Calcium binding tosynaptotagmin-1 participates in triggering neurotransmitter releaseat the synapse (Fernandez-Chacon et al., 2001 [PubMed11242035]).
Background Reference 1:
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Background Reference 2:
Musch, M.W., et al. Am. J. Physiol. Gastrointest. Liver Physiol. 298 (2), G203-G211 (2010) :
Background Reference 3:
Kathir, K.M., et al. Biochim. Biophys. Acta 1798(2):297-302(2010)
Background Reference 4:
Pattaro, C., et al. BMC Med. Genet. 11, 41 (2010) :

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-214

List Size:
400 ul

List Price:

$495.00

Datasheet
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