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Western blot analysis of SYTL2 Antibody in Hela cell line lysates (35ug/lane)
SYTL2 Antibody IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of SYTL2 Antibody in Hela cell line lysates (35ug/lane)

SYTL2 Antibody

CATALOG NUMBER: 61-670

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This SYTL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 840-868 amino acids from the Central region of human SYTL2.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
105 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SYTL2
Additional Names:
Synaptotagmin-like protein 2, Breast cancer-associated antigen SGA-72M, Exophilin-4, SYTL2, KIAA1597, SGA72M, SLP2, SLP2A
Protein Accession Number:
Q9HCH5
Protein Gi Number:
257051068
Ncbi Gene Id Number:
54843
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SYTL2 is a synaptotagmin-like protein (SLP) that belongs to a C2 domain-containing protein family. The SLP homology domain (SHD) of this protein has been shown to specifically bind the GTP-bound form of Ras-related protein Rab-27A (RAB27A). This protein plays a role in RAB27A-dependent vesicle trafficking and controls melanosome distribution in the cell periphery.
Background Reference 1:
Menasche, G., et al. Blood 112(13):5052-5062(2008)
Background Reference 2:
Kesari, A., et al. Am. J. Pathol. 173(5):1476-1487(2008)
Background Reference 3:
Chavas, L.M., et al. Structure 16(10):1468-1477(2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-670

List Size:
400 ul

List Price:

$495.00

Datasheet
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