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Western blot analysis of extracts of various cell lines, using TACR2 antibody (18-947) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
Western blot analysis of extracts of various cell lines, using TACR2 antibody (18-947) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.

TACR2 Antibody

CATALOG NUMBER: 18-947

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 319-398 of human TACR2 (NP_001048.2).
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
WB: 1:200 - 1:1000
Positive Control 1:
SGC-7901
Positive Control 2:
A-549
Predicted Molecular Weight:
Observed: 44kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
TACR2
Additional Names:
TACR2, tachykinin receptor 2, NK2R, NKNAR, SKR, TAC2R, NK-2 receptor, neurokinin 2 receptor, neurokinin A receptor, seven transmembrane helix Receptor, substance K receptor
Ncbi Gene Id Number:
6865
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene belongs to a family of genes that function as receptors for tachykinins. Receptor affinities are specified by variations in the 5'-end of the sequence. The receptors belonging to this family are characterized by interactions with G proteins and 7 hydrophobic transmembrane regions. This gene encodes the receptor for the tachykinin neuropeptide substance K, also referred to as neurokinin A.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

18-947

List Size:
100 uL

List Price:

$436.00

Out of stock

Datasheet
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