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Western Blot at 1:1000 dilution + Ramos whole cell lysate Lysates/proteins at 20 ug per lane.
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western Blot at 1:1000 dilution + Ramos whole cell lysate Lysates/proteins at 20 ug per lane.

TAF4B Antibody

CATALOG NUMBER: 56-253

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This TAF4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 647-675 amino acids from the C-terminal region of human TAF4B.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
91 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TAF4B
Additional Names:
Transcription initiation factor TFIID subunit 4B, Transcription initiation factor TFIID 105 kDa subunit, TAF(II)105, TAFII-105, TAFII105, TAF4B, TAF2C2, TAFII105
Protein Accession Number:
Q92750
Protein Gi Number:
119370533
Ncbi Gene Id Number:
6875
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TATA-binding protein associated factors (TAFs)participate, with TATA binding protein (TBP; MIM 600075), in theformation of the TFIID protein complex (see MIM 313650), which isinvolved in the initiation of gene transcription by RNA polymeraseII (see MIM 180660).
Background Reference 1:
Kalogeropoulou, M., et al. Mol. Cancer Res. 8(4):554-568(2010)
Background Reference 2:
Gazit, K., et al. J. Biol. Chem. 284(39):26286-26296(2009)
Background Reference 3:
Knauff, E.A., et al. Hum. Reprod. 24(9):2372-2378(2009)
Background Reference 4:
Teye, K., et al. Int. J. Oncol. 33(6):1271-1280(2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-253

List Size:
400 ul

List Price:

$495.00

Datasheet
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