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TAF8 Antibody

CATALOG NUMBER: 42-253

Clonality:
Polyclonal
Tested Applications:
ELISA
Host Species:
Goat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Homology:
Expected Species Reactivity based on sequence homology: Human, Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: KSYCEHTARTQPT
Conjugate:
Unconjugated
Tested Applications:
ELISA
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave an approx 38kDa band in Human Brain (Amygdala, Cerebellum, Hippocampus) lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 34.3kDa according to NP_612639.2. The 38kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
TAF8
Additional Names:
TAF8, TAF8 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 43kDa, 43, FLJ32821, II, TAF, TAFII43, TBN, OTTHUMP00000016392, TAF(II)43, TAF8 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 50 kD, TATA box binding protein (TBP)-associated factor, RNA polymerase II, A, 45/50kDa, TBP-associated factor 8, TBP-associated factor TAFII43, TBP-associated factor, RNA polymerase II, 43 kD, taube nuss homolog
Protein Accession Number:
NP_612639.2
Protein Gi Number:
104485446
Ncbi Gene Id Number:
129685
Background Reference 1:
Guermah M, Ge K, Chiang CM, Roeder RG, The TBN protein, which is essential for early embryonic mouse development, is an inducible TAFII implicated in adipogenesis. Molecular cell 2003 Oct 12 (4): 991-1001.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-253

List Size:
0.1 mg

List Price:

$468.00

Datasheet
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