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46-467 (0.3µg/ml) staining of Human Testes lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
46-467 (5ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. <strong>This data is from a previous batch, not on sale.</strong>
46-467 (0.3µg/ml) staining of Human Testes lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

TAGLN Antibody

CATALOG NUMBER: 46-467

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-MTGYGRPRQIIS
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 23-24kDa band observed in Human Testis lysates (calculated MW of 22.6kDa according to NP_003177.2 and NP_001001522.1). Preliminary testing also showed a band at approx 23kDa in lysates of cell line HeLa and approx 25kDa in Human Breast and Placenta lysates Recommended concentration: 0.1-1 µg/ml. Primary incubation 1 hour at room temperature.
Specificity:
NP_003177.2 and NP_001001522.1 represent identical protein.The transgelin protein is found in fibroblasts and smooth muscle and is known as a transformation or shape change protein that is involved with actin cross-linking. Transgelin is one of the earliest markers of differentiated smooth muscle cells and recent evidence suggests that transgelin acts as a tumour suppressor.
Positive Control 1:
Cat. No. 1309 - Human Placenta Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
TAGLN
Additional Names:
TAGLN, SM22, SMCC, WS3-10, transgelin, SM22-alpha, DKFZp686P11128, TAGLN1, DKFZp686B01212, transgelin variant 2
Protein Accession Number:
NP_003177.2
Protein Gi Number:
48255905
Ncbi Gene Id Number:
6876
Background Reference 1:
Nishida W, Kitami Y, Abe M, Hiwada K. Gene cloning and nucleotide sequence of SM22 alpha from the chicken gizzard smooth muscle. Biochem Int. 1991 Mar;23(4):663-8.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-467

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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