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Western blot analysis of TAGLN antibody in mouse bladder tissue lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with TAGLN Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of NCI-H292 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of TAGLN antibody in mouse bladder tissue lysates (35ug/lane)

TAGLN Antibody

CATALOG NUMBER: 62-395

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This TAGLN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 139-167 amino acids from the C-terminal region of human TAGLN.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
23 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TAGLN
Additional Names:
Transgelin, 22 kDa actin-binding protein, Protein WS3-10, Smooth muscle protein 22-alpha, SM22-alpha, TAGLN, SM22, WS3-10
Protein Accession Number:
Q01995
Protein Gi Number:
3123283
Ncbi Gene Id Number:
6876
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TAGLN is a transformation and shape-change sensitive actin cross-linking/gelling protein found in fibroblasts and smooth muscle. Its expression is down-regulated in many cell lines, and this down-regulation may be an early and sensitive marker for the onset of transformation. A functional role of this protein is unclear.
Background Reference 1:
Zhao,L., Mod. Pathol. 22 (6), 786-796 (2009) Leguillette,R., Am. J. Respir. Crit. Care Med. 179 (3), 194-204 (2009) Yu,H., FASEB J. 22 (6), 1778-1789 (2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-395

List Size:
400 ul

List Price:

$495.00

Datasheet
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