Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

Western blot analysis of TAK1 in Rat thymus cell lysate with TAK1 antibody at (A) 1, (B) 2, and (C) 4 μg/mL.
Immunofluorescence of TAK1 in Rat Thymus cells with TAK1 antibody at 10 μg/mL.
Western blot analysis of TAK1 in Rat thymus cell lysate with TAK1 antibody at (A) 1, (B) 2, and (C) 4 μg/mL.
Trial Size

TAK1 Antibody

CATALOG NUMBER: 3385

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
TAK1 antibody was raised against a synthetic peptide corresponding to 14 amino acids near the amino-terminus of human TAK1 isoform a.
The immunogen is located within amino acids 40 - 90 of TAK1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
TAK1 antibody can be used for the detection of TAK1 by Western blot at 1 to 4 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1471 - Rat Thymus Tissue Lysate
Purification:
TAK1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
TAK1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TAK1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MAP3K7
Additional Names:
TAK1 Antibody: TAK1, MEKK7, TGF1a, TAK1, Mitogen-activated protein kinase kinase kinase 7, Transforming growth factor-beta-activated kinase 1, TGF-beta-activated kinase 1
Protein Accession Number:
BAA25025
Protein Gi Number:
2924624
Ncbi Gene Id Number:
6885
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TAK1 Antibody: TAK1 (TGF-beta activated protein kinase 1) is a widely expressed enzyme originally identified as a mitogen-activated protein kinase kinase kinase (MAP3K7). It has since been shown to mediate various intracellular signaling pathways, such as those induced by TGF-beta and members of the Toll-IL-1R (TIR) superfamily, thus acting as an intermediate in both proliferative and innate and adaptive immune responses. TAK1 is normally present in cells in a complex with TAK1-binding protein 1 (TAB1) and either TAB2 or TAB3. Association with TAB1 triggers TAK1 kinase activity by inducing TAK1 autophosphorylation, while TAB2 and possibly TAB3 may contribute to SAPK2a/p38a-mediated feedback inhibition of TAK1 activity.
Background Reference 1:
Yamaguchi K, Shirakabe K, Shibuya H, et al. Identification of a member of the MAPKKK family as a potential mediator of TGF-β signal transduction. Science 1995; 270:2008-11.
Background Reference 2:
Irie T, Muta T, and Takeshige K. TAK1 mediates an activation signal from toll-like receptor(s) to nuclear factor-κB in lipopolysaccharide-stimulated macrophages. FEBS Lett. 2000; 467:160-4.
Background Reference 3:
Akira S and Takeda K. Toll-like receptor Signalling. Nat. Rev. Immunol. 2004; 4:499-511.
Background Reference 4:
Jiang Z, Ninomiya-Tsuji J, Qian Y, et al. Interleukin-1 (IL-1) receptor-associated kinase-dependent IL-1-induced signaling complexes phosphorylate TAK1 and TAB2 at the plasma membrane and activate TAK1 in the cytosol. Mol. Cell Biol. 2002; 22:7158-67.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3385

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369