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Western blot analysis of TAK1 in Rat thymus cell lysate with TAK1 antibody at (A) 1, (B) 2, and (C) 4 μg/mL.
Immunofluorescence of TAK1 in Rat Thymus cells with TAK1 antibody at 10 μg/mL.
Western blot analysis of TAK1 in Rat thymus cell lysate with TAK1 antibody at (A) 1, (B) 2, and (C) 4 μg/mL.
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TAK1 Antibody

CATALOG NUMBER: 3385

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
TAK1 antibody was raised against a synthetic peptide corresponding to 14 amino acids near the amino-terminus of human TAK1 isoform a.
The immunogen is located within amino acids 40 - 90 of TAK1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
TAK1 antibody can be used for the detection of TAK1 by Western blot at 1 to 4 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1471 - Rat Thymus Tissue Lysate
Purification:
TAK1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
TAK1 Antibody is supplied in PBS containing ≤0.09% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TAK1 antibody can be stored at 4°C, stable for up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MAP3K7
Additional Names:
TAK1 Antibody: TAK1, MEKK7, TGF1a, TAK1, Mitogen-activated protein kinase kinase kinase 7, Transforming growth factor-beta-activated kinase 1, TGF-beta-activated kinase 1
Protein Accession Number:
BAA25025
Protein Gi Number:
2924624
Ncbi Gene Id Number:
6885
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TAK1 Antibody: TAK1 (TGF-beta activated protein kinase 1) is a widely expressed enzyme originally identified as a mitogen-activated protein kinase kinase kinase (MAP3K7). It has since been shown to mediate various intracellular signaling pathways, such as those induced by TGF-beta and members of the Toll-IL-1R (TIR) superfamily, thus acting as an intermediate in both proliferative and innate and adaptive immune responses. TAK1 is normally present in cells in a complex with TAK1-binding protein 1 (TAB1) and either TAB2 or TAB3. Association with TAB1 triggers TAK1 kinase activity by inducing TAK1 autophosphorylation, while TAB2 and possibly TAB3 may contribute to SAPK2a/p38a-mediated feedback inhibition of TAK1 activity.
Background Reference 1:
Yamaguchi K, Shirakabe K, Shibuya H, et al. Identification of a member of the MAPKKK family as a potential mediator of TGF-β signal transduction. Science 1995; 270:2008-11.
Background Reference 2:
Irie T, Muta T, and Takeshige K. TAK1 mediates an activation signal from toll-like receptor(s) to nuclear factor-κB in lipopolysaccharide-stimulated macrophages. FEBS Lett. 2000; 467:160-4.
Background Reference 3:
Akira S and Takeda K. Toll-like receptor Signalling. Nat. Rev. Immunol. 2004; 4:499-511.
Background Reference 4:
Jiang Z, Ninomiya-Tsuji J, Qian Y, et al. Interleukin-1 (IL-1) receptor-associated kinase-dependent IL-1-induced signaling complexes phosphorylate TAK1 and TAB2 at the plasma membrane and activate TAK1 in the cytosol. Mol. Cell Biol. 2002; 22:7158-67.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3385

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

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