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46-469 (1ug/ml) staining of HeLa (A) and U937 (B) and (2ug/ml) Daudi (C) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-469 (2ug/ml) staining of NIH3T3 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-469 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fol
46-469 (1ug/ml) staining of HeLa (A) and U937 (B) and (2ug/ml) Daudi (C) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

TAK1 Antibody

CATALOG NUMBER: 46-469

Clonality:
Polyclonal
Tested Applications:
ELISA, Flow, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Rat, Cow
Immunogen:
The immunogen for this antibody is: C-AELDQDEKDQQNT
Conjugate:
Unconjugated
Tested Applications:
ELISA, Flow, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 75kDa band observed in lysates of cell lines HeLa, Daudi and NIH3T3, and approx. 80kDa in lysates of cell line U937 (calculated MW of 67.2 according to Human NP_663304.1 and Mouse NP_033342.1). The observed molecular weight corresponds to different antibodies from other commercial sources. Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature.Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
Specificity:
This antibody is expected to recognise isoform A (NP_003179.1) and isoform B (NP_663304.1).
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
MAP3K7
Additional Names:
MAP3K7 , TAK1, mitogen-activated protein kinase kinase kinase 7 , TGF1a , TGF-beta activated kinase 1, transforming growth factor-beta-activated kinase 1
Protein Accession Number:
NP_003179.1,NP_663304.1
Protein Gi Number:
4507361
Ncbi Gene Id Number:
6885
Background Reference 1:
Omori E, Matsumoto K, Sanjo H, Sato S, Akira S, Smart RC, Ninomiya-Tsuji J. TAK1 is a master regulator of epidermal homeostasis involving skin inflammation and apoptosis. J Biol Chem. 2006 May 4;

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-469

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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