TAO2 (phospho Ser181) Antibody

CATALOG NUMBER: XPS-1029

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
TAO2 (Ser181) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho Ser181 of TAO2.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
Human, mouse, rat and Xenopus have 100% amino acid sequence identity with the antigen used to raise the antibody. Applications include Dot Blots (DB) and Western Blots (WB). Suitability for Immunohistochemistry (IHC) has not been determined. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.
Specificity:
TAO2 antibody is specific for Mr ~120k TAO2 phosphorylated at Ser181.
Predicted Molecular Weight:
120
Purification:
Affinity Purified
Clonality:
Polyclonal
Physical State:
Liquid
Concentration:
batch dependent
Storage Conditions:
For long term storage –80°C is recommended, but shorter term storage at –20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.
Ncbi Official Symbol:
TAOK2
Protein Accession Number:
Q9UL54
Protein Gi Number:
116242813
Ncbi Gene Id Number:
9344
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TAO (thousand and one amino acid) protein kinases mediate signaling from carbachol to p38 MAPK and ternary complex transcription factors. This has led to the proposal that the TAO protein kinases play an essential role in signaling from physiological agonists to the stress-responsive p38 MAPKs. Autophosphorylation of TAO may play a role in the mechanism of TAO activation. The MEK binding domain of TAO is autophosphorylated on both serine and threonine residues and Ser181 is located within this domain.
Background Reference 1:
Chen, z., Raman, M., Chen, L., Lee, S.F., Gilman, A.G., and Cobb, M.H., "TAO (thousand-and-one amino acid) protein kinases mediate signaling from carbachol to p38 mitogen-activated protein kinase and ternary complex factors," J. Biol. Chem. 278 (2003) 22,278-22,283.
Background Reference 2:
Chen z., and Cobb, M.H., "Regulation of stress-responsive mitogen-activated protein (MAP) kinase pathways by TAO2", J. Biol. Chem. 276 (2001) 16,070-16,075.
Background Reference 3:
Chen, Z., Hutchison, M., and Cobb, M.H., "Isolation of the protein kinase TAO2 and identification of its mitogen-activated protein kinase/extracellular signal-regulated kinase kinase binding domain," J. Biol. Chem. 274 (1999) 28,803-28,807.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

XPS-1029

List Size:
0.1 mL

List Price:

$540.00

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