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Western blot analysis of extracts of various cell lines, using TARDBP antibody (14-156) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human esophagus using TARDBP Antibody (14-156) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human endometrium using TARDBP Antibody (14-156) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of HeLa cells using TARDBP antibody (14-156). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of SW620 cells using 3ug TARDBP antibody (14-156). Western blot was performed from the immunoprecipitate using TARDBP antibody (14-156) at a dilition of 1:500.
Western blot analysis of extracts of various cell lines, using TARDBP antibody (14-156) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

TARDBP Antibody

CATALOG NUMBER: 14-156

Clonality:
Polyclonal
Tested Applications:
IF, IHC, IP, RIP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human TARDBP (NP_031401.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, IP, RIP, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:100
IF: 1:20 - 1:100
IP: 1:50 - 1:100
RIP: 1:20 - 1:50
Positive Control 1:
HeLa
Positive Control 2:
Jurkat
Positive Control 3:
SW620
Positive Control 4:
HT-29
Positive Control 5:
A-673
Positive Control 6:
Es-2
Predicted Molecular Weight:
Observed: 42kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
TARDBP
Additional Names:
TDP43 Antibody: ALS10, TDP-43, TDP43, TAR DNA-binding protein 43
Ncbi Gene Id Number:
23435
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

14-156

List Size:
100 uL

List Price:

$436.00

Datasheet
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