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46-778 (0.3ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
46-778 (1ug/ml) staining of Human Cerebellum (A), Frontal Cotex (B) and Hippocampus (C) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
46-778 (0.3ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

TARDBP Antibody

CATALOG NUMBER: 46-778

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-HISNAEPKHNSNRQ
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx. 45kDa band observed in lysates of cell lines Jurkat (calculated MW of 44.7kDa according to NP_031401.1). A slightly larger protein is detected in some regions of the Brain. Recommended concentration: 0.3-1ug/ml.
Positive Control 1:
Cat. No. 1375 - Human Skeletal Muscle Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
TARDBP
Additional Names:
TARDBP, TAR DNA binding protein, ALS10, TDP-43, OTTHUMP00000002171, TAR DNA-binding protein-43
Protein Accession Number:
NP_031401.1
Protein Gi Number:
6678271
Ncbi Gene Id Number:
23435
Background Reference 1:
Zhang YJ, Xu YF, Dickey CA, Buratti E, Baralle F, Bailey R, Pickering-Brown S, Dickson D, Petrucelli L. Progranulin mediates caspase-dependent cleavage of TAR DNA binding protein-43. J. Neurosci. 2007 Sep 27 (39): 10530-4.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-778

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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