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Western blot of rat brain homogenate showing specific immunolabeling of the ~59, 65, 68k Tau isoforms phosphorylated at Ser416 (control). Immunolabeling is blocked by preadsorption with the phospho-peptide used as antigen (Peptide) but not by the corresponding dephospho-peptide (not shown).
Western blot of rat brain homogenate showing specific immunolabeling of the ~59, 65, 68k Tau isoforms phosphorylated at Ser416 (control). Immunolabeling is blocked by preadsorption with the phospho-peptide used as antigen (Peptide) but not by the corresponding dephospho-peptide (not shown).

Tau (phospho Ser416) Antibody

CATALOG NUMBER: 50-270

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Bovine, Chicken, Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Bovine, Chicken, Human, Mouse, Rat
Immunogen:
Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser416 of rat tau.
Conjugate:
Unconjugated
Tested Applications:
WB
Specificity:
Specific for ~59, 65, 68k tau protein phosphorylated at Ser416.
Predicted Molecular Weight:
59/65/68
Purification:
Affinity Purified
Clonality:
Polyclonal
physical-state:
Liquid
Concentration:
batch dependent
Storage Conditions:
Tau (Ser416) antibody can be stored at -20°C and is stable at -20°C for at least 1 year.
Ncbi Official Symbol:
Mapt
Protein Accession Number:
P19332
Protein Gi Number:
13432197
Ncbi Gene Id Number:
29477
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Tau is a key microtubule-associated protein that plays an important role in the formation of microtubules in axons (Binder et al. 1985). Six tau isoforms have been identified as products of a single gene produced by alternative mRNA splicing (Goedert 1990). Tau mutations have been implicated in many neurodegenerative disorders such as Alzheimer’s disease (AD), Pick’s disease and progressive supranuclear palsy. It has been well documented that hyperphosphorylated tau is a major component of paired helical filaments in AD brain (Lee 1995). Serine 416 has been demonstrated to be a major phosphorylation site in vitro by CaM kinase II (Steiner at al. 1990).
Background Reference 1:
Binder LI, Frankfurter A, Rebhun LI (1985) The distribution of tau in the mammalian central nervous system. J Cell Bio Oct; 101(4):1371-8.
Background Reference 2:
Lee V.M.Y. (1995) Disruption of the cytoskeleton in Alzheimer’s disease. Curr. Opin. Neurobiol. 5, 663-668.
Background Reference 3:
Goedert M. and Jakes R. (1990) Expression of separate isoforms of human tau protein: correlation with the tau pattern in brain and effects on tubulin polymerization. EMBO J 9, 4225-4230.
Background Reference 4:
Steiner B., Mandelkow E.M., Biernat J. et al. (1990) Phosphorylation of microtubule-associated protein tau: identification of the site for Ca2+-calmodulin dependent kinase and relationship with tau phosphorylation in Alzheimer tangles. EMBO J. 9, 3539-3544.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

50-270

List Size:
0.1 mL

List Price:

$540.00

Datasheet
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