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Western Blot at 1:1000 dilution + LNCaP whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in mouse kidney tissue lysates (35ug/lane).
TBCA Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human rectum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western Blot at 1:1000 dilution + LNCaP whole cell lysate Lysates/proteins at 20 ug per lane.

TBCA Antibody

CATALOG NUMBER: 56-183

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This TBCA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30-58 amino acids from the Central region of human TBCA.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
13 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TBCA
Additional Names:
Tubulin-specific chaperone A, TCP1-chaperonin cofactor A, Tubulin-folding cofactor A, CFA, TBCA
Protein Accession Number:
O75347
Ncbi Gene Id Number:
6902
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The product of this gene is one of four proteins(cofactors A, D, E, and C) involved in the pathway leading tocorrectly folded beta-tubulin from folding intermediates. CofactorsA and D are believed to play a role in capturing and stabilizingbeta-tubulin intermediates in a quasi-native confirmation. CofactorE binds to the cofactor D/beta-tubulin complex; interaction withcofactor C then causes the release of beta-tubulin polypeptidesthat are committed to the native state. This gene encodeschaperonin cofactor A.
Background Reference 1:
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Background Reference 2:
Joslyn, G., et al. Alcohol. Clin. Exp. Res. 34(5):800-812(2010)
Background Reference 3:
Bruneel, A., et al. Proteomics 5(15):3876-3884(2005)
Background Reference 4:
Nolasco, S., et al. FEBS Lett. 579(17):3515-3524(2005)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-183

List Size:
400 ul

List Price:

$495.00

Datasheet
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