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Western blot analysis of extracts of various cell lines, using TBL1XR1 antibody (23-975) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 20s.
Western blot analysis of extracts of various cell lines, using TBL1XR1 antibody (23-975) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 20s.

TBL1XR1 Antibody [7G7D7]

CATALOG NUMBER: 23-975

Clonality:
Monoclonal
Tested Applications:
ICC, IHC, WB
Host Species:
Mouse
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human, Mouse
Immunogen:
Recombinant protein of human TBL1XR1
Conjugate:
Unconjugated
Tested Applications:
ICC, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
ICC: 1:50 - 1:200
Positive Control 1:
K-562
Positive Control 2:
293T
Positive Control 3:
HeLa
Positive Control 4:
NIH/3T3
Positive Control 5:
HT-1080
Positive Control 6:
THP-1
Predicted Molecular Weight:
Observed: 55kDa
Purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
TBL1XR1
Additional Names:
F-box-like/WD repeat-containing protein TBL1XR1, Nuclear receptor corepressor/HDAC3 complex subunit TBLR1, TBL1-related protein 1, Transducin beta-like 1X-related protein 1, TBL1XR1, IRA1, TBLR1
Ncbi Gene Id Number:
79718
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene is a member of the WD40 repeat-containing gene family and shares sequence similarity with transducin (beta)-like 1X-linked (TBL1X). The protein encoded by this gene is thought to be a component of both nuclear receptor corepressor (N-CoR) and histone deacetylase 3 (HDAC 3) complexes, and is required for transcriptional activation by a variety of transcription factors. Mutations in these gene have been associated with some autism spectrum disorders, and one finding suggests that haploinsufficiency of this gene may be a cause of intellectual disability with dysmorphism. Mutations in this gene as well as recurrent translocations involving this gene have also been observed in some tumors.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

23-975

List Size:
50 uL

List Price:

$335.00

Out of stock

Datasheet
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