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42-998 (1ug/ml) staining of HeLa nuclear (A) and cytosolic (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-998 (1ug/ml) staining of cell line NIH3T3 (A), Mouse Testis (B) and Rat Testis (C) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
42-998 (1ug/ml) staining of Pig Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
42-998 (1ug/ml) staining of HeLa nuclear (A) and cytosolic (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

TBP Antibody

CATALOG NUMBER: 42-998

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Homology:
Expected Species Reactivity based on sequence homology: Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-TPQPIQNTNSLS
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 40kDa band observed in nuclear, but not in cytosolic lysates of cell line HeLa (calculated MW of 37.7kDa according to NP_003185.1) and 37kDa observed in lysates of NIH3T3 and of Mouse and Rat Testis lysates (calculated MW of 34.7kDa according to Mouse NP_038712.3 and of 35.0kDa according to Rat NP_001004198.1). Recommended concentration: 1-3ug/ml.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_003185.1; NP_001165556.1).
Predicted Molecular Weight:
Approx 40 kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
TBP
Additional Names:
TBP, TATA box binding protein, GTF2D, GTF2D1, HDL4, MGC117320, MGC126054, MGC126055, SCA17, TFIID, TATA sequence-binding protein, TATA-box binding protein N-terminal domain, TATA-box factor, TATA-box-binding protein, transcription initiation factor TFIID TBP subunit
Protein Accession Number:
NP_003185.1,NP_001165556.1
Protein Gi Number:
4507379
Ncbi Gene Id Number:
6908

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-998

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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