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Immunohistochemical analysis of paraffin-embedded H. breast section using TBX1 Antibody . Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Western blot analysis of lysate from HLVEC cell line, using TBX1 Antibody at 1:1000 at each lane.
Western blot analysis in HLVEC cell line lysates (35ug/lane).
Fluorescent confocal image of A2058 cell stained with TBX1 Antibody . A2058 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TBX1 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). TBX1 immunoreactivity is localized to nucleus significantly.
Formalin-fixed and paraffin-embedded human testis tissue reacted with TBX1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Immunohistochemical analysis of paraffin-embedded H. breast section using TBX1 Antibody . Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

TBX1 Antibody

CATALOG NUMBER: 63-920

Clonality:
Polyclonal
Tested Applications:
IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Tested Applications:
IF, IHC-P, WB
Application Note:
For IHC-P starting dilution is: 1:25
For WB starting dilution is: 1:1000
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
43 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TBX1
Additional Names:
T-box transcription factor TBX1, T-box protein 1, Testis-specific T-box protein, TBX1
Protein Accession Number:
O43435
Ncbi Gene Id Number:
6899
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TBX1 is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene product shares 98% amino acid sequence identity with the mouse ortholog. DiGeorge syndrome (DGS)/velocardiofacial syndrome (VCFS), a common congenital disorder characterized by neural-crest-related developmental defects, has been associated with deletions of chromosome 22q11.2, where this gene has been mapped. Studies using mouse models of DiGeorge syndrome suggest a major role for this gene in the molecular etiology of DGS/VCFS. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene.
Background Reference 1:
Fernando, R.I., et al. J. Clin. Invest. 120(2):533-544(2010)
Background Reference 2:
Heike, C.L., et al. Birth Defects Res. PartClin. Mol. Teratol. 88(1):54-63(2010)
Background Reference 3:
Beaujard, M.P., et al. EurMed Genet 52(5):321-327(2009)
Background Reference 4:
Shalaby, A.A., et al. Mod. Pathol. 22(8):996-1005(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-920

List Size:
400 ul

List Price:

$495.00

Datasheet
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