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Antibody used in IHC on Human Skeletal Muscle.
Antibody used in IHC on Human kidney.
Antibody used in WB on Human Liver at 0.2-1 ug/ml.
Antibody used in IHC on Human Skeletal Muscle.

TBX21 Antibody

CATALOG NUMBER: 27-646

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TBX21.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
TBX21 antibody can be used for detection of TBX21 by ELISA at 1:312500. TBX21 antibody can be used for detection of TBX21 by western blot at 1.0-2.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1304 - Human Liver Tissue Lysate
Predicted Molecular Weight:
58 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store TBX21 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
TBX21
Additional Names:
TBX21, HGNC:11599, T-PET, T-bet, TBLYM, TBET
Protein Accession Number:
NP_037483
Protein Gi Number:
7019549
Ncbi Gene Id Number:
30009
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TBX21 is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. It is the human ortholog of mouse Tbx21/Tbet gene. Studies in mouse show that Tbx21 protein is a Th1 cell-specific transcription factor that controls the expression of the hallmark Th1 cytokine, interferon-gamma (IFNG). Expression of the human ortholog also correlates with IFNG expression in Th1 and natural killer cells, suggesting a role for this gene in initiating Th1 lineage development from naive Th precursor cells.
Background Reference 1:
Sasaki, Y., et al., (2004) Hum. Genet. 115 (3), 177-184.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

27-646

List Size:
100 ul

List Price:

$519.00

Datasheet
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