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Antibody used in IHC on Human Brain, cortex at 5.0 ug/ml.
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
Antibody used in IHC on Human Brain, cortex at 5.0 ug/ml.

TBX5 Antibody

CATALOG NUMBER: 27-850

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat, Zebrafish
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat, Zebrafish
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TBX5.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
TBX5 antibody can be used for detection of TBX5 by ELISA at 1:62500. TBX5 antibody can be used for detection of TBX5 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Predicted Molecular Weight:
58 kDa, 60 kDa, 53 kDa, 39 kDa, 58 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store TBX5 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
TBX5
Additional Names:
TBX5, HOS
Protein Accession Number:
NP_000183
Protein Gi Number:
18201892
Ncbi Gene Id Number:
6910
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TBX5 is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. TBX5 is closely linked to related family member T-box 3 (ulnar mammary syndrome) on human chromosome 12. The encoded protein may play a role in heart development and specification of limb identity. Mutations in TBX5 have been associated with Holt-Oram syndrome, a developmental disorder affecting the heart and upper limbs. Several transcript variants encoding different isoforms have been described for TBX5.
Background Reference 1:
Zaragoza, M.V., et al., (2004) Gene 330, 38978.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

27-850

List Size:
100 ul

List Price:

$519.00

Datasheet
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