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Western blot analysis of TCC52 in 293 cell lysate with TCC52 antibody at 1 μg/mL.
Immunocytochemistry of TCC52 in 293 cells with TCC52 antibody at 2.5 μg/mL.
Western blot analysis of TCC52 in 293 cell lysate with TCC52 antibody at 1 μg/mL.
Trial Size

TCC52 Antibody

CATALOG NUMBER: 5703

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, WB
Host Species:
Chicken
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Chicken
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%), Chicken: (87%)
Immunogen:
TCC52 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human TCC52.
The immunogen is located within amino acids 390 - 440 of TCC52.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, WB
Application Note:
TCC52 antibody can be used for detection of TCC52 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1210 - HEK293 Cell Lysate
Positive Control 2:
Cat. No. 17-010 - HEK293 Cell Slide
Purification:
TCC52 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgY
physical-state:
Liquid
Buffer:
TCC52 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TCC52 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DCAF12
Additional Names:
TCC52 Antibody: CT102, TCC52, WDR40A, KIAA1892, DDB1- and CUL4-associated factor 12, Centrosome-related protein TCC52
Protein Accession Number:
NP_056212
Protein Gi Number:
22218619
Ncbi Gene Id Number:
25853
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TCC52 Antibody: Cancer-testis (CT) antigens are a type of antigen whose expression is highly restricted, primarily in normal testis, but aberrantly in different tumor types. Their immunogenicity in cancer patients makes them prime targets for immunotherapy of human tumors. One such CT antigen is TCC52, a novel centrosomal protein. TCC52 is a WD repeat-containing protein that interacts with the COP9 signalosome, a macromolecular complex that interacts with cullin-RING E3 ligases and regulates their activity by hydrolyzing cullin-Nedd8 conjugates. The normal biological function of this protein is not known at this time.
Background Reference 1:
Scanlan M, Simpson A, and Old L. The cancer/testis genes: review, standardization, and commentary. Cancer Immun.2004; 4:1-15.
Background Reference 2:
Xia L, Li Y, Yang D, et al. Identification of new centrosome proteins by autoimmune patient sera. Sci. China C. Life Sci.2007; 50:194-202.
Background Reference 3:
Li S, Hu X, Cui S, et al. Novel centrosome protein, TCC52, is a cancer-testis antigen. Cancer Sci.2008; 11:2274-9.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5703

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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