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Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: PC-3 whole cell lysate Lysates/proteins at 20 ug per lane.
TCEB1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: PC-3 whole cell lysate Lysates/proteins at 20 ug per lane.

TCEB1 Antibody

CATALOG NUMBER: 58-012

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Rat
Immunogen:
This TCEB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 69-98 amino acids from the C-terminal region of human TCEB1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
12 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TCEB1
Additional Names:
Transcription elongation factor B polypeptide 1, Elongin 15 kDa subunit, Elongin-C, EloC, RNA polymerase II transcription factor SIII subunit C, SIII p15, TCEB1
Protein Accession Number:
Q15369
Protein Gi Number:
32699511
Ncbi Gene Id Number:
6921
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes the protein elongin C, which is asubunit of the transcription factor B (SIII) complex. The SIIIcomplex is composed of elongins A/A2, B and C. It activateselongation by RNA polymerase II by suppressing transient pausing ofthe polymerase at many sites within transcription units. Elongin Afunctions as the transcriptionally active component of the SIIIcomplex, whereas elongins B and C are regulatory subunits. ElonginA2 is specifically expressed in the testis, and capable of forminga stable complex with elongins B and C. The von Hippel-Lindau tumorsuppressor protein binds to elongins B and C, and thereby inhibitstranscription elongation.
Background Reference 1:
Marcsisin, S.R., et al. J. Mol. Biol. 402(5):892-904(2010)
Background Reference 2:
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Background Reference 3:
Lievens, S., et al. J. Proteome Res. 8(2):877-886(2009)
Background Reference 4:
Jalava, S.E., et al. Int. J. Cancer 124(1):95-102(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

58-012

List Size:
400 ul

List Price:

$495.00

Datasheet
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