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Western blot analysis of TCF12 in HeLa cell lysate with TCF12 antibody at (A) 0.5 and (B) 1 μg/mL.
Immunocytochemistry of TCF12 in HeLa cells with TCF12 antibody at 10 μg/mL.
Western blot analysis of TCF12 in HeLa cell lysate with TCF12 antibody at (A) 0.5 and (B) 1 μg/mL.
Trial Size

TCF12 Antibody

CATALOG NUMBER: 5999

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Chicken: (95%)
Immunogen:
TCF12 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human TCF12.
The immunogen is located within amino acids 50 - 100 of TCF12.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, WB
Application Note:
TCF12 antibody can be used for detection of TCF12 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Positive Control 2:
Cat. No. 17-001 - HeLa Cell Slide
Purification:
TCF12 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
TCF12 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TCF12 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TCF12
Additional Names:
TCF12 Antibody: HEB, CRS3, HTF4, bHLHb20, HsT17266, BHLHB20, HEB, Transcription factor 12, Class B basic helix-loop-helix protein 20, TCF-12
Protein Accession Number:
AAH50556
Protein Gi Number:
29792012
Ncbi Gene Id Number:
6938
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TCF12 Antibody: TCF12, also known as HTF4, is a member of the basic helix-loop-helix (bHLH) E-protein family that recognizes the consensus binding site (E-box) CANNTG. TCF12 is expressed in many tissues, among them skeletal muscle, thymus, B- and T-cells, and may participate in regulating lineage-specific gene expression through the formation of heterodimers with other bHLH E-proteins. TCF12, in combination with E2A, is required to block thymocyte proliferation prior to pre-TCR expression and is critical for proper T cell differentiation. Recent reports have shown that TCF12 is also a critical factor required for the development of invariant natural killer T cells.
Background Reference 1:
Zhang Y and Bina M. The nucelotide sequence of the human transcription factor HTF4a cDNA. DNA Seq.1992; 2:397-403.
Background Reference 2:
Murre C, McCaw PS, Vaesin H, et al. Interactions between heterologous helix-loop-helix proteins generate complexes that bind specifically to a common DNA sequence. Cell1989; 58:537-44.
Background Reference 3:
Wojciechowski J, Lai A, Kondo M, et al. E2A and HEB are required to block thymocyte proliferation prior to pre-TCR expression. J. Immunol.2007; 178:5717-26.
Background Reference 4:
D'Cruz LM, Knell J, Fujimoto JK, et al. An essential role for the transcription factor HEB in thymocyte survival, Tcra rearrangement and the development of natural killer T cells. Nat. Immunol.2010; 11:240-9.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5999

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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