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Western blot analysis of lysates from Hela cell line mouse spleen tissue lysate(from left to right), using TCF21 Antibody at 1:1000 at each lane.
Western blot analysis in 293 cell line and human placenta tissue lysates (35ug/lane).
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of lysates from Hela cell line mouse spleen tissue lysate(from left to right), using TCF21 Antibody at 1:1000 at each lane.

TCF21 Antibody

CATALOG NUMBER: 55-924

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This TCF21 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 144-173 amino acids from the C-terminal region of human TCF21.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
20 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TCF21
Additional Names:
Transcription factor 21, TCF-21, Capsulin, Class A basic helix-loop-helix protein 23, bHLHa23, Epicardin, Podocyte-expressed 1, Pod-1, TCF21, BHLHA23, POD1
Protein Accession Number:
O43680
Ncbi Gene Id Number:
6943
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TCF21 encodes a transcription factor of the basichelix-loop-helix family. The TCF21 product is mesoderm specific,and expressed in embryonic epicardium, mesenchyme-derived tissuesof lung, gut, gonad, and both mesenchymal and glomerular epithelialcells in the kidney. Two transcript variants encoding the sameprotein have been found for this gene.
Background Reference 1:
Jugessur, A., et al. PLoS ONE(7), E11493 (2010) :
Background Reference 2:
Vieira, A.R., et al. Genet. Med. 10(9):668-674(2008)
Background Reference 3:
Melzer, D., et al. PLoS Genet.(5), E1000072 (2008) :
Background Reference 4:
Smith, L.T., et al. Proc. Natl. Acad. Sci. U.S.A. 103(4):982-987(2006)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-924

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

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