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Western blot analysis of TCF3 in Human brain tissue lysate with TCF3 antibody at 1 μg/mL in (A) the absence and (B) presence of peptide blocking.
Immunohistochemistry of TCF3 in rat liver tissue with TCF3 antibody at 5 μg/mL.
Immunofluorescence of TCF3 in rat brain tissue with TCF3 antibody at 20 μg/mL.
Western blot analysis of TCF3 in Human brain tissue lysate with TCF3 antibody at 1 μg/mL in (A) the absence and (B) presence of peptide blocking.
Trial Size

TCF3 Antibody

CATALOG NUMBER: 5609

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Chicken: (86%)
Immunogen:
TCF3 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human TCF3.
The immunogen is located within amino acids 150 - 200 of TCF3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
TCF3 antibody can be used for detection of TCF3 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1303 - Human Brain Tissue Lysate
Purification:
TCF3 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
TCF3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TCF3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TCF3
Additional Names:
TCF3 Antibody: E2A, E47, ITF1, VDIR, TCF-3, bHLHb21, BHLHB21, E2A, Transcription factor E2-alpha, Class B basic helix-loop-helix protein 21
Protein Accession Number:
NP_003191
Protein Gi Number:
27777636
Ncbi Gene Id Number:
6929
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TCF3 Antibody: The TCF3 gene, also called E2A, encodes two basic helix-loop-helix (bHLH) transcription factors, E12 and E47, through alternative splicing. These transcription factors are involved in mediating canonical Wnt signaling, which is very important in a diverse array of cellular functions such as stem cell proliferation, self-renewal, activation, fate determination, differentiation and aging and senescence. They bind beta-catenin and can act as transcriptional activators or repressors for Wnt target genes, and have been shown to regulate specific target genes during CNS development downstream of Wnt signaling. TCF3/Lef complexes are also known to play key roles in controlling cell fate lineages in multipotent skin stem cells.
Background Reference 1:
Korinek V, Barker N, Willert K, et al. Two members of the Tcf family implicated in Wnt/beta-catenin signaling during embryogenesis in the mouse. Mol. Cell Biol.1998; 18:1248-1256.
Background Reference 2:
Gribble SL, Kim HS, Bonner J, et al. Tcf3 inhibits spinal cord neurogenesis by regulating sox4a expression. Dev. Cell2009; 136:781-9.
Background Reference 3:
Cole MF, Johnstone SE, Newman JJ, et al. Tcf3 is an integral component of the core regulatory circuitry of embryonic stem cells. Genes Dev.2008;22:746-55.
Background Reference 4:
Nguyen H, Rendl M and Fuchs E. Tcf3 governs stem cell features and represses cell fate determination in skin. Cell2006; 127:171-83.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5609

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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