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Western Blot at 1:2000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution Lane 1: HCT116 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: mouse cerebellum lysate Lane 5: mouse heart lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in mouse cerebellum tissue lysates (35ug/lane).
Western Blot at 1:2000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.

TCF7L2 Antibody

CATALOG NUMBER: 56-412

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This TCF7L2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 61-90 amino acids from the N-terminal region of human TCF7L2.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:2000
Predicted Molecular Weight:
68 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TCF7L2
Additional Names:
Transcription factor 7-like 2, HMG box transcription factor 4, T-cell-specific transcription factor 4, T-cell factor 4, TCF-4, hTCF-4, TCF7L2, TCF4
Protein Accession Number:
Q9NQB0
Protein Gi Number:
29337146
Ncbi Gene Id Number:
6934
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a high mobility group (HMG)box-containing transcription factor that plays a key role in theWnt signaling pathway. The protein has been implicated in bloodglucose homeostasis. Genetic variants of this gene are associatedwith increased risk of type 2 diabetes. Several transcript variantsencoding multiple different isoforms have been found for this gene.
Background Reference 1:
Hansson, O., et al. Curr. Diab. Rep. 10(6):444-451(2010)
Background Reference 2:
Heni, M., et al. Diabetes (2010) In press :
Background Reference 3:
Potapov, V.A., et al. Genetika 46(8):1123-1131(2010)
Background Reference 4:
Kucharska-Newton, A.M., et al.Obes 2010 (2010) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-412

List Size:
400 ul

List Price:

$495.00

Datasheet
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