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Western blot analysis of TCHP Antibody in CEM cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human skin tissue reacted with TCHP Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of TCHP Antibody in CEM cell line lysates (35ug/lane)

TCHP Antibody

CATALOG NUMBER: 63-907

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This TCHP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 291-320 amino acids from the Central region of human TCHP.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
61 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TCHP
Additional Names:
Trichoplein keratin filament-binding protein, Protein TCHP, Mitochondrial protein with oncostatic activity, Mitostatin, Tumor suppressor protein, TCHP {ECO:0000312, EMBL:AAH042851}
Protein Accession Number:
Q9BT92
Protein Gi Number:
74733103
Ncbi Gene Id Number:
84260
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TCHP may act as a 'capping' or 'branching' protein for keratin filaments in the cell periphery. This protein may regulate K8/K18 filament and desmosome organization mainly at the apical or peripheral regions of simple epithelial cells.
Background Reference 1:
Vecchione,A. Oncogene 28 (2), 257-269 (2009)
Background Reference 2:
Tokumasu,F. J. Cell. Sci. 118 (PT 5), 1091-1098 (2005)
Background Reference 3:
Nishizawa,M. J. Cell. Sci. 118 (PT 5), 1081-1090 (2005)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-907

List Size:
400 ul

List Price:

$495.00

Datasheet
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