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42-011 (0.01ug/ml) staining of Mouse Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-011 (5ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-011 (0.01ug/ml) staining of Mouse Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

TCP1 Antibody

CATALOG NUMBER: 42-011

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Rat, Pig, Cow, Dog
Immunogen:
The immunogen for this antibody is: C-GHKKKGDDQSRQGG
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 65kDa band observed in Mouse Testis lysates (calculated MW of 60.5kDa according to Human NP_005989.3 and 60.6kDa according to Mouse NP_033966.1 and Rat NP_954522.1). Recommended concentration: 0.01-0.03ug/ml.Immunohistochemistry:Paraffin embedded Human Tonsil. Recommended concentration: 5ug/ml.
Specificity:
This antibody is expected to recognize all reported isoforms (NP_005989.3; NP_001008883.1; NP_001008800.1).
Positive Control 1:
Cat. No. 1416 - Mouse Testis Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
CCT3
Additional Names:
CCT3, chaperonin containing TCP1, subunit 3 (gamma), CCT-gamma, CCTG, PIG48, TCP-1-gamma, TRIC5, OTTHUMP00000025735, T-complex protein 1, gamma subunit, TCP1 (t-complex-1) ring complex, polypeptide 5, chaperonin containing TCP1, subunit 3
Protein Accession Number:
NP_005989.3,NP_001008883.1,NP_001008800.1
Protein Gi Number:
63162572
Ncbi Gene Id Number:
7203
Background Reference 1:
Barbe L, Lundberg E, Oksvold P, Stenius A, Lewin E, Björling E, Asplund A, Ponten F, Brismar H, Uhlen M, Andersson-Svahn H. Toward a confocal subcellular atlas of the human proteome. Mol Cell Proteomics. 2008 Mar;7(3):499-508.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-011

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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