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Western blot analysis of TCTEX1D3 in EL4 cell lysate with TCTEX1D3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunofluorescence of TCTEX1D3 in human testis tissue with TCTEX1D3 antibody at 20 μg/mL.
Western blot analysis of TCTEX1D3 in EL4 cell lysate with TCTEX1D3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Trial Size

TCTEX1D3 Antibody

CATALOG NUMBER: 6955

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
TCTEX1D3 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human TCTEX1D3.
The immunogen is located within the first 50 amino acids of TCTEX1D3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
TCTEX1D3 antibody can be used for detection of TCTEX1D3 by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
TCTEX1D3 is predicted to not cross-react with other members of the TCTEX-type family
Positive Control 1:
Cat. No. 1287 - EL4 Cell Lysate
Positive Control 2:
Cat. No. 11-701 - Human Testis Tissue Slide
Predicted Molecular Weight:
22 kDa
Purification:
TCTEX1D3 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
TCTEX1D3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TCTEX1D3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TCTE3
Additional Names:
TCTEX1D3 Antibody: TCTEX1D3, TCTEX1D3, Tctex1 domain-containing protein 3, T-complex testis-specific protein 3, Tcte-3
Protein Accession Number:
NP_777570
Protein Gi Number:
28372535
Ncbi Gene Id Number:
6991
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TCTEX1D3 Antibody: Dyneins are multi-subunit, high molecular weight ATPases that interact with microtubules to generate force by converting the chemical energy of ATP into the mechanical energy of movement. Cytoplasmic or axonemal Dynein is an approximately 12 subunit complex of two heavy chains, two intermediate chains to anchor Dynein to its cargo, four smaller intermediate chains and several light chains. It performs functions necessary for cell survival such as organelle transport and centrosome assembly. TCTEX1 is a cytoplasmic dynein light chain found in a complex with Na+ CP type X (SCN10A). TCTEX1D3, also called TCTE3, has been identified as a gene responsible for congenital diaphragmatic hernia (CDH).
Background Reference 1:
He Y, Francis F, Myers KA, et al. Role of cytoplasmic dynein in the axonal transport of microtubules and neurofilaments. J. Cell Biol. 2005; 168:697-703.
Background Reference 2:
Li J, Lee WL, and Cooper JA. NudEL targets dynein to microtubule ends through LIS1. Nat. Cell Biol. 2005; 7:686-90.
Background Reference 3:
Watanabe TK, Fujiwara T, Shimizu F, et al. Cloning, expression, and mapping of TCTEL1, a putative human homologue of murine Tcte1 to 6q. Cytogenet. Cell Genet. 1996; 73:153-6.
Background Reference 4:
Chuang JZ, Yeh TY, Bollati F, et al. The dynein light chain Tctex-1 has a dynein-independent role in actin remodeling during neurite outgrowth. Dev. Cell 2005; 9:75-86.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6955

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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