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Western blot analysis of TDG antibody in K562 cell line lysates (35ug/lane)
Flow cytometric analysis of K562 cells using TDG Antibody (bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of TDG antibody in K562 cell line lysates (35ug/lane)

TDG Antibody

CATALOG NUMBER: 62-366

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This TDG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 281-310 amino acids from the C-terminal region of human TDG.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
46 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TDG
Additional Names:
G/T mismatch-specific thymine DNA glycosylase, Thymine-DNA glycosylase, hTDG, TDG
Protein Accession Number:
Q13569
Protein Gi Number:
46397791
Ncbi Gene Id Number:
6996
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein TDG belongs to the TDG/mug DNA glycosylase family. Thymine-DNA glycosylase (TDG) removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine.
Background Reference 1:
Kim,E.J., Biochem. Biophys. Res. Commun. 377 (3), 838-842 (2008)
Background Reference 2:
Neddermann,P., J. Biol. Chem. 271 (22), 12767-12774 (1996)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-366

List Size:
400 ul

List Price:

$495.00

Datasheet
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