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Western blot analysis of TDGF1 in 293 cell lysate with TDGF1 antibody at 1 μg/mL.
Immunofluorescence of TDGF1 in 293 cells with TDGF1 antibody at 20 μg/mL.
Western blot analysis of TDGF1 in 293 cell lysate with TDGF1 antibody at 1 μg/mL.
Trial Size

TDGF1 Antibody

CATALOG NUMBER: 7099

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Rabbit polyclonal TDGF1 antibody was raised against a 17 amino acid peptide near the amino terminus of human TDGF1.
The immunogen is located within amino acids 40 - 90 of TDGF1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
TDGF1 antibody can be used for detection of TDGF1 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1210 - HEK293 Cell Lysate
Positive Control 2:
Cat. No. 17-010 - HEK293 Cell Slide
Predicted Molecular Weight:
21 kDa
Purification:
TDGF1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
TDGF1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TDGF1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TDGF1
Additional Names:
TDGF1 Antibody: CR, CRGF, CRIPTO, Teratocarcinoma-derived growth factor 1, Cripto-1 growth factor
Protein Accession Number:
NP_003203
Protein Gi Number:
4507425
Ncbi Gene Id Number:
6997
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TDGF1 Antibody: Teratocarcinoma-derived growth factor 1 (TDGF1 or Cripto) is a member of the epidermal growth factor-cripto FRL1 cryptic protein family and is involved in the activation of several different signaling pathways during embryonic development and cellular transformation. It is first expressed in the forming mesoderm during gastrulation but later in development the expression is restricted to the truncus arteriosus of the developing heart. This suggests that TDGF1 mediates the progression of epiblastic cells that give rise to the mesoderm. TDGF1 overexpression is characteristic of human gastric and colorectal carcinomas.
Background Reference 1:
Brandt R, Normanno N, Gullick WJ, et al. Identification and biological characterization of an epidermal growth factor-related protein: Cripto-1. J. Biol. Chem. 1994; 269:17320-8.
Background Reference 2:
Dono R, Scalera L, Pacifico F, et al. The murine Cripto gene: expression during mesoderm induction and early heart morphogenesis. Development 1993; 118:1157-68.
Background Reference 3:
Parisi S, D'Andrea D, Lago CT, et al. Nodal-dependent Cripto signaling promotes cardiomyogenesis and redirects the neural fate of embryonic stem cells. J. Cell Biol. 2003; 163:303-14.
Background Reference 4:
Xing PX, Hu XF, Pietersz GA, et al. Cripto: a novel target for antibody-based cancer immunotherapy. Cancer Res. 2004; 64:4018-23.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7099

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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