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43-543 (2ug/ml) staining of Jurkat nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-543 (2ug/ml) staining of Jurkat nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

TDP1 Antibody

CATALOG NUMBER: 43-543

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-PNDGTAQRTENHG
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 75kDa band observed in nuclear lysates of cell line Jurkat (calculated MW of 68.4kDa according to NP_060789.2). Recommended concentration: 1-3ug/ml.
Specificity:
Reported variants represent identical protein: NP_001008744.1, NP_060789.2
Predicted Molecular Weight:
Approx 75kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
TDP1
Additional Names:
TDP1, tyrosyl-DNA phosphodiesterase 1, FLJ11090, MGC104252
Protein Accession Number:
NP_060789.2
Protein Gi Number:
37999797
Ncbi Gene Id Number:
55775
Background Reference 1:
Antony S, Marchand C, Stephen AG, Thibaut L, Agama KK, Fisher RJ, Pommier YNovel high-throughput electrochemiluminescent assay for identification of human tyrosyl-DNA phosphodiesterase (Tdp1) inhibitors and characterization of furamidine (NSC 305831) as an inhibitor of Tdp1

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-543

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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