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Western blot analysis in A549 cell line lysates (35ug/lane).
Western blot analysis in mouse bladder tissue lysates (35ug/lane).
TDRD7 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in A549 cell line lysates (35ug/lane).

TDRD7 Antibody

CATALOG NUMBER: 55-541

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Rat
Immunogen:
This TDRD7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 807-835 amino acids from the C-terminal region of human TDRD7.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
124 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TDRD7
Additional Names:
Tudor domain-containing protein 7, PCTAIRE2-binding protein, Tudor repeat associator with PCTAIRE-2, Trap, TDRD7, PCTAIRE2BP
Protein Accession Number:
Q8NHU6
Protein Gi Number:
152031705
Ncbi Gene Id Number:
23424
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TDRD7, also known as Tudor domain containing protein 7, is a component of chromatoid bodies in spermatids (named processing bodies or P-bodies in somatic cells). It is found in a mRNP complex together with TDRD1, TDRD6 and DDX4. It is also found in a complex with CABLES1, PCTK2 and PIWIL1. TDRD7 has been shown to interact with histone H3 tri-methylated at K9 in vitro.
Background Reference 1:
Lim, J., et al. Cell 125(4):801-814(2006)
Background Reference 2:
Conte, N., et al. Oncogene 22(50):8102-8116(2003)
Background Reference 3:
Lauffart, B., et al. Biochem. J. 363 (PT 1), 195-200 (2002) :
Background Reference 4:
Yamochi, T., et al. Biochem. Biophys. Res. Commun. 286(5):1045-1050(2001)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-541

List Size:
400 ul

List Price:

$495.00

Datasheet
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