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Western blot analysis in K562 cell line lysates (35ug/lane).
Western blot analysis in K562 cell line lysates (35ug/lane).

TdT Antibody

CATALOG NUMBER: 55-915

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This TdT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 438-467 amino acids from the C-terminal region of human TdT.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
59 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DNTT
Additional Names:
DNA nucleotidylexotransferase, Terminal addition enzyme, Terminal deoxynucleotidyltransferase, Terminal transferase, DNTT, TDT
Protein Accession Number:
P04053
Protein Gi Number:
311033533
Ncbi Gene Id Number:
1791
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene is a member of the DNA polymerase type-X familyand encodes a template-independent DNA polymerase that catalyzesthe addition of deoxynucleotides to the 3'-hydroxyl terminus ofoligonucleotide primers. In vivo, the encoded protein is expressedin a restricted population of normal and malignant pre-B and pre-Tlymphocytes during early differentiation, where it generatesantigen receptor diversity by synthesizing non-germ line elements(N-regions) at the junctions of rearranged Ig heavy chain and Tcell receptor gene segments. Alternatively spliced transcriptvariants encoding different isoforms of this gene have beendescribed.
Background Reference 1:
Kubota, T., et al. Genes Cells 12(8):941-959(2007)
Background Reference 2:
O'Malley, D.P., et al. Haematologica 91(8):1139-1140(2006)
Background Reference 3:
Grupe, A., et al. Am. J. Hum. Genet. 78(1):78-88(2006)
Background Reference 4:
Thai, T.H., et al. J. Immunol. 173(6):4009-4019(2004)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-915

List Size:
400 ul

List Price:

$495.00

Datasheet
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