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Western blot analysis of lysates from wild type (WT) and 293T cells transfected with TEAD2 using TEAD1/2/3/4 Rabbit mAb(A5092) at 1:1000 dilution incubated overnight at 4℃.  | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 20 s.
Western blot analysis of lysates from wild type (WT) and 293F cells transfected with TEAD3 using TEAD1/2/3/4 Rabbit mAb(A5092) at 1:1000 dilution incubated overnight at 4℃.  | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 10 s.
Western blot analysis of various lysates using TEAD1/2/3/4 Rabbit mAb (A5092) at 1:3000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 1 min.
Western blot analysis of lysates from wild type (WT) and 293T cells transfected with TEAD2 using TEAD1/2/3/4 Rabbit mAb(A5092) at 1:1000 dilution incubated overnight at 4℃.  | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 20 s.

TEAD1/2/3/4 Rabbit Recombinant Antibody

CATALOG NUMBER: 84-088

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
WB,1:1000 - 1:5000, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
293T transfected with TEAD2 (Human), 293F transfected with TEAD3 (Human), 293T transfected with TEAD1 (Human), 293T transfected with TEAD4 (Human)
Predicted Molecular Weight:
48,48,49,47kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
TEAD1/2/3/4
Additional Names:
AA; REF1; TCF13; TEF-1; NTEF-1; TCF-13; TEAD-1
Protein Accession Number:
P28347/Q15561/Q15562/Q99594
Ncbi Gene Id Number:
7003/7004/8463/7005
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a ubiquitous transcriptional enhancer factor that is a member of the TEA/ATTS domain family. This protein directs the transactivation of a wide variety of genes and, in placental cells, also acts as a transcriptional repressor. Mutations in this gene cause Sveinsson's chorioretinal atrophy. Additional transcript variants have been described but their full-length natures have not been experimentally verified.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

84-088

List Size:
100 uL

List Price:

$477.00

Datasheet
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