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Western blot analysis of various lysates using TEAD1 Rabbit mAb (A5218) at 1:1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 10s.
Immunoprecipitation analysis of 600 μg extracts of Mouse skeletal muscle cells using 3 μg TEAD1 antibody (A5218). Western blot was performed from the immunoprecipitate using TEAD1 antibody (A5218) at a dilution of 1:1000.
Chromatin immunoprecipitation analysis of extracts of HepG2 cells, using TEAD1 antibody (A5218) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina(RK20265) from 10⁵ Hep G2 cells with 1 μg TEAD1 Rabbit mAb (A5218), along with a Goat Anti-Rabbit IgG(H+L). The CUT&Tag results indicate the enrichment pattern of TEAD1 in representative gene loci (SLC7A5), as shown in figure.
Western blot analysis of various lysates using TEAD1 Rabbit mAb (A5218) at 1:1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 10s.

TEAD1 Rabbit Recombinant Antibody

CATALOG NUMBER: 84-131

Clonality:
Monoclonal
Tested Applications:
ChIP, CUT&Tag, ELISA, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ChIP, CUT&Tag, ELISA, IP, WB
Application Note:
WB,1:500 - 1:2000, IP,0.5μg-4μg antibody for 400μg-600μg extracts of whole cells, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements., ChIP,5μg antibody for 10μg-15μg of Chromatin, CUT&Tag, 10⁵ cells /1 μg
Positive Control 1:
HeLa,Mouse skeletal muscle,Mouse heart,Rat skeletal muscle,Rat heart
Predicted Molecular Weight:
48kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
TEAD1
Additional Names:
AA; REF1; TCF13; TEF-1; NTEF-1; TCF-13; TEAD-1; TEAD1
Protein Accession Number:
P28347
Ncbi Gene Id Number:
7003
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a ubiquitous transcriptional enhancer factor that is a member of the TEA/ATTS domain family. This protein directs the transactivation of a wide variety of genes and, in placental cells, also acts as a transcriptional repressor. Mutations in this gene cause Sveinsson's chorioretinal atrophy. Additional transcript variants have been described but their full-length natures have not been experimentally verified.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

84-131

List Size:
100 uL

List Price:

$540.00

Datasheet
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