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Antibody used in WB on Transfected 293T at 0.2-1 ug/ml.
Antibody used in IF on HeLa at 4 ug/ml.
Antibody used in WB on Transfected 293T at 0.2-1 ug/ml.

TEAD4 Antibody

CATALOG NUMBER: 25-140

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TEAD4.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
TEAD4 antibody can be used for detection of TEAD4 by ELISA at 1:12500. TEAD4 antibody can be used for detection of TEAD4 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Tranfected 293T Cell Lysate
Predicted Molecular Weight:
44 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store TEAD4 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
TEAD4
Additional Names:
TEAD4, EFTR-2, MGC9014, RTEF1, TCF13L1, TEF-3, TEFR-1, hRTEF-1B, TEF3
Protein Accession Number:
NP_958849
Protein Gi Number:
46411156
Ncbi Gene Id Number:
7004
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TEAD4 is a member of the transcriptional enhancer factor (TEF) family of transcription factors, which contain the TEA/ATTS DNA-binding domain. TEAD4 is preferentially expressed in the skeletal muscle, and binds to the M-CAT regulatory element found in promoters of muscle-specific genes to direct their gene expression. Alternatively spliced transcripts encoding distinct isoforms, some of which are translated through the use of a non-AUG (UUG) initiation codon, have been described for this protein.This gene product is a member of the transcriptional enhancer factor (TEF) family of transcription factors, which contain the TEA/ATTS DNA-binding domain. It is preferentially expressed in the skeletal muscle, and binds to the M-CAT regulatory element found in promoters of muscle-specific genes to direct their gene expression. Alternatively spliced transcripts encoding distinct isoforms, some of which are translated through the use of a non-AUG (UUG) initiation codon, have been described for this gene.
Background Reference 1:
Appukuttan, B., (2007) Invest. Ophthalmol. Vis. Sci. 48 (8), 3775-3782.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

25-140

List Size:
100 ul

List Price:

$519.00

Datasheet
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