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Western blot analysis of TEKT5 in 3T3 cell lysate with TEKT5 antibody at (A) 0.25 and (B) 0.5 μg/mL.
Immunohistochemistry of TEKT5 in rat testis tissue with TEKT5 antibody at 5 μg/mL.
Western blot analysis of TEKT5 in 3T3 cell lysate with TEKT5 antibody at (A) 0.25 and (B) 0.5 μg/mL.
Trial Size

TEKT5 Antibody

CATALOG NUMBER: 6383

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (88%)
Immunogen:
TEKT5 antibody was raised against a 17 amino acid synthetic peptide near the center of human TEKT5.
The immunogen is located within amino acids 220 - 270 of TEKT5.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, WB
Application Note:
TEKT5 antibody can be used for detection of TEKT5 by Western blot at 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunohistochemistry in rat samples. All other applications and species not yet tested.
Specificity:
This antibody is predicted to not cross-react with other Tektin protein family members
Positive Control 1:
Cat. No. 1282 - 3T3 (NIH) Cell Lysate
Purification:
TEKT5 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
TEKT5 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TEKT5 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TEKT5
Additional Names:
TEKT5 Antibody: CT149, Tektin-5
Protein Accession Number:
NP_653275
Protein Gi Number:
21389569
Ncbi Gene Id Number:
146279
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TEKT5 Antibody: Tektins are insoluble alpha-helical proteins related to intermediate filament proteins and are essential for the assembly of cilia and flagella. Tektin-5 (TEKT5) is a recently identified member of the Tektin family. It is predominantly expressed in testis, and its expression is upregulated during testis development. It is present in sperm flagella but not in heads, suggesting that it may act as a middle component required for flagellar stability and sperm mobility.
Background Reference 1:
Amos LA. The tektin family of microtubule-stabilizing proteins. Genome Biol. 2008; 9:229.
Background Reference 2:
Murayama E, Yamamoto E, Kaneko T, et al. Tektin5, a new Tektin family member, is a component of the middle piece of flagella in rat spermatozoa. Mol. Reprod. Dev. 2008; 75:650-8.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6383

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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