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Western blot analysis of TEM2 in rat colon tissue lysate with TEM2 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of TEM2 in human colon tissue with TEM2 antibody at 2.5 μg/mL.
Immunofluorescence of TEM2 in Human Colon cells with TEM2 antibody at 20 μg/mL.
Western blot analysis of TEM2 in rat colon tissue lysate with TEM2 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

TEM2 Antibody

CATALOG NUMBER: 4363

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
TEM2 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of the human TEM2.
The immunogen is located within amino acids 20 - 70 of TEM2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
TEM2 antibody can be used for detection of TEM2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
TEM2 antibody may cross-react with the closely related protein RASD1.
Positive Control 1:
Cat. No. 1472 - Rat Colon Tissue Lysate
Purification:
TEM2 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
TEM2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TEM2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
RASD2
Additional Names:
TEM2 Antibody: Rhes, TEM2, MGC:4834, GTP-binding protein Rhes, Ras homolog enriched in striatum
Protein Accession Number:
Q96D21
Protein Gi Number:
21362868
Ncbi Gene Id Number:
23551
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TEM2 Antibody: Rhes, also known as tumor endothelial marker 2 (TEM2), is a small GTP-binding protein that is predominantly expressed in the striatal region of the brain. This protein belongs to the RASD subfamily of Ras-related GTP-binding protein superfamily and is thought to play a role in the normal development and function of the brain as mice lacking this gene showed increased anxiety levels and motor coordination deficits. Rhes was identified as TEM2 through analysis of genes whose expression was upregulated in tumor endothelium. Tumor endothelial markers are significantly up-regulated during angiogenesis and neoangiogenesis that are crucial for the growth of solid tumors. TEMs localized on the cell surface and conserved across species are of particular interest for future development of anti-angiogenic therapies.
Background Reference 1:
Vargiu P, Morte B, Manzano J, et al. Thyroid hormone regulation of rhes, a novel RAS homolog gene expressed in the striatum. Brain Res. Mol. Brain Res.2001; 94:1-8.
Background Reference 2:
Spano D, Branchi I, Rosica A, et al. Rhes is involved in striatal function. Mol. Cell. Biol.2004; 24:5788-96.
Background Reference 3:
Carson-Walter EB, Watkins DN, Nanda A, et al. Cell surface tumor endothelial markers are conserved in mice and humans. Cancer Res.2001; 61:6649-55.
Background Reference 4:
Yamamoto Y, Irie K, Nanda A, et al. Direct binding of the human homologue of the Drosophila disc large tumor suppressor gene to seven-pass transmembrane proteins, tumor endothelial marker 5 (TEM5), and a novel TEM5-like protein. Oncogene2004; 23:3889-97.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4363

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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