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Western blot analysis of TEM5 in rat kidney tissue lysate with TEM5 antibody at (A) 2 μg/ml and (B) 4 μg/mL.
Immunohistochemistry of TEM5 in human bladder tissue with TEM5 antibody at 5 μg/mL.
Immunofluorescence of TEM5 in human bladder tissue with TEM5 antibody at 20 μg/mL.
Western blot analysis of TEM5 in rat kidney tissue lysate with TEM5 antibody at (A) 2 μg/ml and (B) 4 μg/mL.
Trial Size

TEM5 Antibody

CATALOG NUMBER: 4371

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (85%)
Immunogen:
TEM5 antibody was raised against a 14 amino acid synthetic peptide near the center of human TEM5.
The immunogen is located within amino acids 560 - 610 of TEM5.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
TEM5 antibody can be used for detection of TEM5 by Western blot at 2 and 4 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1465 - Rat Kidney Tissue Lysate
Purification:
TEM5 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
TEM5 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TEM5 antibody can be stored at 4°C, stable for up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
GPR124
Additional Names:
TEM5 Antibody: TEM5, KIAA1531, TEM5, G-protein coupled receptor 124, Tumor endothelial marker 5
Protein Accession Number:
Q96PE1
Protein Gi Number:
221222450
Ncbi Gene Id Number:
25960
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TEM5 Antibody: Tumor endothelial markers (TEMs) are significantly up-regulated during angiogenesis and neoangiogensis that are crucial for the growth of solid tumors. TEMs localized on the cell surface and conserved across species are of particular interest for future development of anti-angiogenic therapies. These include TEMs such as TEM1, TEM5, TEM7 and TEM8. TEM5 is a member of the adhesion family of G protein coupled receptors and is localized on the surface of endothelial cells. TEM5 is a seven-pass transmembrane receptor, unlike TEM1, TEM7 and TEM8 which span the membrane once. TEM5 is abundantly expressed in tumor vessels, heart, placenta, ovary, small intestine, and colon. Proteolytically processed soluble TEM5 mediates endothelial cell survival during angiogenesis by linking integrin to glycosaminoglycans.
Background Reference 1:
Carson-Walter EB, Watkins DN, Nanda A, et al. Cell surface tumor endothelial markers are conserved in mice and humans. Cancer Res.2001; 61:6649-55.
Background Reference 2:
Yamamoto Y, Irie K, Nanda A, et al. Direct binding of the human homologue of the Drosophila disc large tumor suppressor gene to seven-pass transmembrane proteins, tumor endothelial marker 5 (TEM5), and a novel TEM5-like protein. Oncogene2004; 23:3889-97.
Background Reference 3:
Davies G, Cunnick GH, Mansel RE, et al. Levels of expression of endothelial markers specific to tumour-associated endothelial cells and their correlation with prognosis in patients with breast cancer. Clinical & Experimental Metastasis2004; 21:31-7.
Background Reference 4:
Vallon M and Essler M. Proteolytically processed soluble tumor endothelial marker (TEM) 5 mediates endothelial cell survival during angiogenesis by linking integrin alpha(v)beta3 to glycosaminoglycans. J. Biol. Chem.2006; 281:34179-88.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4371

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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