Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

Western blot analysis of TEM7 in rat liver tissue lysate with TEM7 antibody at (A) 0.5 and (B) 1 μg/mL.
Immunohistochemistry of TEM7 in mouse liver tissue with TEM7 antibody at 2.5 μg/mL.
Immunofluorescence of TEM7 in Mouse Liver cells with TEM7 antibody at 20 μg/mL.
Western blot analysis of TEM7 in rat liver tissue lysate with TEM7 antibody at (A) 0.5 and (B) 1 μg/mL.
Trial Size

TEM7 Antibody

CATALOG NUMBER: 4341

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
TEM7 antibody was raised against a 16 amino acid synthetic peptide from near the center of human TEM7.
The immunogen is located within amino acids 250 - 300 of TEM7.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
TEM7 antibody can be used for detection of TEM7 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1464 - Rat Liver Tissue Lysate
Purification:
TEM7 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
TEM7 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TEM7 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PLXDC1
Additional Names:
TEM7 Antibody: TEM3, TEM7, TEM3, Plexin domain-containing protein 1, Tumor endothelial marker 3
Protein Accession Number:
AAG00869
Protein Gi Number:
14017379
Ncbi Gene Id Number:
57125
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TEM7 Antibody: Tumor endothelial marker 7 (TEM7) was identified through serial analysis of gene expression on endothelial cells isolated from human normal and malignant colorectal tissues. Further experiments verified TEM7 was highly expressed in the endothelium of numerous other cancer types including breast, lung and brain tumors. At least four isoforms of TEM7 are known to exist; these include intracellular, secreted, and membrane-bound forms. A homologous protein, TEM7R (also known as PLXDC2), acts as a binding partner to TEM7 and is also abundantly expressed in the endothelium of malignant colorectal cancer but is absent or rare in normal colon mucosa. High expression of TEM7 is associated with metastasis and poor survival of patients with osteogenic sarcoma.
Background Reference 1:
St. Croix B, Rago C, Velculescu V, et al. Genes expressed in human tumor endothelium. Science2000; 289:1197-202.
Background Reference 2:
Nanda A, Buckhaults P, Seaman S, et al. Identification of a binding partner for the endothelial cell surface proteins TEM7 and TEM7R. Cancer Res.2004; 64:8507-11.
Background Reference 3:
Carson-Weber EB, Watkins DN, Nanda A, et al. Cell surface tumor epithelial markers are conserved in mice and humans. Cancer Res.2001; 61:6649-55.
Background Reference 4:
Fuchs B, Mahlum E, Halder C, et al. High expression of tumor endothelial marker 7 is associated with metastasis and poor survival of patients with osteogenic sarcoma. Gene2007; 399:137-43.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4341

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369