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Western blot analysis of TENM1 in human brain tissue lysate with TENM1 antibody at 1 μg/ml.
Immunohistochemistry of TENM1 in mouse brain tissue with TENM1 antibody at 5 μg/mL.
Immunofluorescence of TENM1 in mouse brain tissue with TENM1 antibody at 20 μg/mL.
Western blot analysis of TENM1 in human brain tissue lysate with TENM1 antibody at 1 μg/ml.
Trial Size

TENM1 Antibody

CATALOG NUMBER: 8149

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Chicken: (83%)
Immunogen:
TENM1 antibody was raised against an 18 amino acid peptide near the amino terminus of human TENM1.
The immunogen is located within the first 50 amino acids of TENM1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
TENM1 antibody can be used for detection of TENM1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity:
TENM1 antibody is human, mouse and rat reactive. TENM1 antibody is predicted to not cross-react with other members of the TENM family.
Positive Control 1:
Cat. No. 1303 - Human Brain Tissue Lysate
Predicted Molecular Weight:
Predicted: 301 kDa
Observed: 280 kDa
Purification:
TENM1 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
TENM1 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TENM1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
TENM1
Additional Names:
Teneurin transmembrane protein 1, teneurin-1, ODZ1, ODZ3, TEN-M1, TNM, TNM1
Protein Accession Number:
NP_001156750
Protein Gi Number:
253970444
Ncbi Gene Id Number:
10178
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The teneurin transmembrane protein 1 (TENM1) is a member of a family of four neuronal cell surface proteins homologous to the Drosophila pair-rule gene Ten-m (1,2). TENM1 is expressed primarily in the developing central nervous system and may be proteolytically cleaved with the intracellular domain translocating to the nucleus (3). TENM1 is a direct target of the homeobox transcription factor EMX2, a transcription factor thought to be important for area specification in the developing cortex (4).
Background Reference 1:
Minet AD, Rubin BP, Tucker RP, et al. Teneurin-1, a vertebrate homologue of the Drosophila pair-rule gene ten-m, is a neuronal protein with a novel type of heparin-binding domain. J. Cell Sci. 1999; 112:2019-32.
Background Reference 2:
Rubin BP, Tucker RP, Martin D, et al. Tenurins: a novel family of neuronal cell surface proteins in vertebrates, homologous to the Drosophila pair-rule gene Ten-m. Dev. Biol. 1999; 216:195-209.
Background Reference 3:
Kenzelmann D, Chiquet-Ehrismann R, Leachman NT, et al. Teneurin-1 is expressed in interconnected regions of the developing brain and processed in vivo. BMC Dev. Biol. 2008; 8:30.
Background Reference 4:
Beckmann J, Vitobello A, Ferralli J, et al. Human teneruin-1 is a direct target of the homeobox transcription factor EMX2 at a novel alternate promoter. BMC Dev. Biol. 2011; 11:35.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

8149

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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