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<strong>WB Validation in 293 Cells</strong><br> Loading: 15 μg of Cell lysate Antibodies: TENM3 8151-8152-2402, 2 μg/mL, 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>WB Validation in Mouse and Rat Brain</strong><br> Loading: 15 µg of Cell lysate Antibodies: TENM3 8151-8152-2402, 2 μg/mL, 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
Immunohistochemistry of TENM3 in mouse brain tissue with TENM3 antibody at 5 μg/mL.
Immunofluorescence of TENM3 in mouse brain tissue with TENM3 antibody at 20 μg/mL.
WB Validation in 293 Cells
Loading: 15 μg of Cell lysate Antibodies: TENM3 8151-8152-2402, 2 μg/mL, 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
Trial Size

TENM3 Antibody

CATALOG NUMBER: 8151

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
TENM3 antibody was raised against a 17 amino acid peptide near the amino terminus of human TENM3.
The immunogen is located within the first 50 amino acids of TENM3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
TENM3 antibody can be used for detection of TENM3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity:
TENM3 antibody is human, mouse and rat reactive. TENM3 antibody is predicted to not cross-react with other members of the TENM family.
Positive Control 1:
Cat. No. 1210 - HEK293 Cell Lysate
Predicted Molecular Weight:
Predicted: 297 kDa
Observed: 300 kDa
Purification:
TENM3 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
TENM3 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TENM3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
TENM3
Additional Names:
Teneurin transmembrane protein 3, teneurin-3, ODZ3, TEN-M3, TNM3, MCOPCB9
Protein Accession Number:
NP_001073946
Protein Gi Number:
122937400
Ncbi Gene Id Number:
55714
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The teneurin transmembrane protein 3 (TENM3) is a member of a family of four neuronal cell surface proteins homologous to the Drosophila pair-rule gene Ten-m (1,2). TENM3 is expressed in cartilaginous cells during postnatal growth in mice (3), as well as the adult eye and optic stalk (4). Homozygous null mutations in TENM3 causes microphthalmia in humans (5).
Background Reference 1:
Ben-Zur T and Wides R. Mapping homologs of Drosophila odd oz (odz): Doc4/Odz to mouse chromosome 7, Odz1 to mouse chromosome 11; and ODZ3 to human chromosome Xq25. Genomics 1999; 58:102-3.
Background Reference 2:
Rubin BP, Tucker RP, Martin D, et al. Tenurins: a novel family of neuronal cell surface proteins in vertebrates, homologous to the Drosophila pair-rule gene Ten-m. Dev. Biol. 1999; 216:195-209.
Background Reference 3:
Murakami T, Fukunaga T, Takeshita N, et al. Expression of Ten-m/Odz3 in the fibrous layer of madibular condylar cartilage during postnatal growth in mice. J. Anat. 2010; 217:236-44.
Background Reference 4:
Ben-Zur T, Feige E, Motro B, et al. The mammalian Odz gene family: homologs of a Drosophila pair-rule gene with expression implying distinct yet overlapping developmental roles. Dev. Biol. 2000; 217:107-20.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

8151

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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