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Western Blot at 1:500 dilution + HL-60 whole cell lysate Lysates/proteins at 20 ug per lane.
Formalin-fixed and paraffin-embedded human testis tissue reacted with TESK2 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western Blot at 1:500 dilution + HL-60 whole cell lysate Lysates/proteins at 20 ug per lane.

TESK2 Antibody

CATALOG NUMBER: 63-217

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This TESK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 210-240 amino acids from the Central region of human TESK2.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:500
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
64 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TESK2
Additional Names:
Dual specificity testis-specific protein kinase 2, Testicular protein kinase 2, TESK2
Protein Accession Number:
Q96S53
Protein Gi Number:
25009462
Ncbi Gene Id Number:
10420
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TESK2 is a dual specificity protein kinase activity catalyzing autophosphorylation and phosphorylation of exogenous substrates on both serine/threonine and tyrosine residues. TESK2 phosphorylates cofilin at Ser-3. It may play an important role in spermatogenesis. This nuclear enzyme is thought to be activated by autophosphorylation on Ser-219. Expression is predominantly in testis and prostate, with highest concentrations in nongerminal Sertoli cells.
Background Reference 1:
Toshima,J.,J. Biol. Chem. 276 (33), 31449-31458 (2001)
Background Reference 2:
Rosok,O., Genomics 61 (1), 44-54 (1999)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-217

List Size:
400 ul

List Price:

$495.00

Datasheet
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