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Western blot analysis of TESPA1 in A431 cell lysate with TESPA1 antibody at 1 μg/ml.
Immunocytochemistry of TESPA1 in A431 cells with TESPA1 antibody at 5 μg/mL.
Immunofluorescence of TESPA1 in A431 cells with TESPA1 antibody at 20 μg/mL.
Western blot analysis of TESPA1 in A431 cell lysate with TESPA1 antibody at 1 μg/ml.
Trial Size

TESPA1 Antibody

CATALOG NUMBER: 7601

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (100%)
Immunogen:
TESPA1 antibody was raised against an 18 amino acid peptide near the amino terminus of human TESPA1.
The immunogen is located within amino acids 20 - 70 of TESPA1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
TESPA1 antibody can be used for detection of TESPA1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
TESPA1 antibody is human specific. At least three isoforms TESPA1 are known to exist.
Positive Control 1:
Cat. No. 1202 - A431 Cell Lysate
Positive Control 2:
Cat. No. 17-002 - A-431 Cell Slide
Predicted Molecular Weight:
Predicted: 57 kDa
Observed: 60 kDa
Purification:
TESPA1 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
TESPA1 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TESPA1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
TESPA1
Additional Names:
TESPA1 Antibody: HSPC257, KIAA0748, HSPC257, Protein TESPA1, Thymocyte-expressed positive selection-associated protein 1
Protein Accession Number:
NP_001129502
Protein Gi Number:
209862861
Ncbi Gene Id Number:
9840
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TESPA1 is a critical component of the TCR signalosome and is essential for T cell selection and maturation through the regulation of TCR signaling during T cell development (1). TESPA1 encodes a protein of 458 amino acids and is highly conserved among vertebrate species (2). TESPA1 expression was tightly regulated during T cell development, with highest expression at the DP stage (3). TESPA1 deficiency resulted in failure of positive selection and substantially fewer of CD4+ and CD8+ SP cells in the thymus (1,4).
Background Reference 1:
Wang D, Zheng M, Lei L, et al. Tespa1 is involved in late thymocyte development through the regulation of TCR-mediated signaling. Nat. Immunol. 2012; 13:560-8.
Background Reference 2:
Matsuzaki H, Fujimoto T, Ota T, et al. Tespa1 is a novel inositol 1,4,5-trisphosphate receptor binding protein in T and B lymphocytes. FEBS Open Bio. 2012; 2:255-9.
Background Reference 3:
Matsuzaki H, Fujimoto T, Tanaka M, et al. Tespa1 is a novel component of mitochondria-associated endoplasmic reticulum membranes and affects mitochondrial calcium flux. Biochem. Biophys. Res. Commun. 2013; 433:322-6.
Background Reference 4:
Fujimoto T, Matsuzaki H, Tanaka M, et al. Tespa1 protein is phosphorylated in response to store-operated calcium entry. Biochem. Biophys. Res. Commun. 2013; 434:162-5.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7601

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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